目的建立以吡嗪酰胺为内标，高效毛细管电泳定量血浆中盐酸川芎嗪的分析方法。方法用未涂层熔融石英毛细管(75μm×60cm，有效长度为50cm)分离，背景电解质为100mmol·L-1SDS-30mmol·L-1硼砂缓冲液（40∶60），分离电压为20kV，检测波长为295nm，毛细管温度为20℃，进样条件3.448kPa。结果分析方法的线性范围是1.12～286.72mg·L-1（r=0.9996），日内精密度RSD值小于4.21%，日间精密度RSD值小于4.74%，平均回收率是91.22%～97.48%，检测限是0.16mg·L-1。结论该方法快速、准确，适合生物样品中盐酸川芎嗪的定量分析。
Abstract
OBJECTIVE To develop an HPCE method of tetramethylpyrazine hydrochloride in rat plasma using pyramide as the internal standard. METHODS The separation was performed on a fused-silica uncoated capillary with an inner diameter (ID) of 75 μm, an effective length of 50 cm and a total length of 60 cm. The optimum HPCE conditions were as follows;100 mmol·L-1 SDS-30 mmol·L-1 sodium tetraborate solution (40;60) was used as the background electrolyt, the samples were injected at the anodic end at 0.5 psi for 6 s, separation voltage was 20 kV at positive power, capillary temperature was 20 ℃, and detection wavelength was 295 nm. RESULTS The linear range was 1.12-286.72 mg·L-1（r=0.999 6）,and the inter-day and intra-day precisions expressed as RSDs were less than 4.21% and 4.74%, respectively. The average recoveries were 91.22%-97.48%, and the limit of detection was 0.16 mg·L-1. CONCLUSION This method is rapid and accurate to quantify tetramethylpyrazine hydrochloride in rat plasma.
关键词
高效毛细管电泳 /
生物样品 /
盐酸川芎嗪 /
吡嗪酰胺doi：10.11669/cpj.2013.09.016
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Key words
HPCE /
bio-sample /
tetramethylpyrazine hydrochloride /
pyramide
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中图分类号：
R969.1
R917
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参考文献
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脚注
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基金
国家自然科学基金资助项目（30500668）
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